Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 2 HI Antibody levels and the specific IgG levels induced by L. lactis HA1-Fc. A Antibody titers determined by HI assay after the chickens were vaccinated with PBS, L. lactis, L. lactis HA1, L. lactis HA1-Fc and inactivated H9N2 vaccine. B The antigen specific IgG titers of chickens after vaccination detected by indirect ELISA. The data were showed as means ± SD (n = 5). **P < 0.01 relative to PBS and L. lactis. ##P < 0.01 relative to L. lactis HA1

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: HI Assay, Indirect ELISA

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 3 Mucosal antibody levels induced by L. lactis HA1-Fc. SIgA antibodies in the feces (A) and BALF (B) were assessed by ELISA after the chickens were vaccinated with PBS, L. lactis, L. lactis HA1, L. lactis HA1-Fc and inactivated H9N2 vaccine. The data were showed as means ± SD (n = 5). ▲▲P < 0.01 relative to PBS. **P < 0.01 relative to PBS and L. lactis. ##P < 0.01 relative to L. lactis HA1. $$P < 0.01 relative to L. lactis HA1-Fc

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 5 T cells proliferation assay. T cells proliferation was examined by Cell Proliferation ELISA BrdU Kit, and results were expressed as a stimulation index (SI) of chickens vaccinated with PBS, L. lactis, L. lactis HA1, L. lactis HA1-Fc and inactivated H9N2 vaccine. The data were showed as means ± SD (n = 5). **P < 0.01 relative to PBS and L. lactis. ##P < 0.01 relative to L. lactis HA1. $$P < 0.01 relative to L. lactis HA1-Fc

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Proliferation Assay, Enzyme-linked Immunosorbent Assay

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 4 IL-2, IL-4, and IFN-γ levels in intestinal tissues induced by L. lactis HA1-Fc. Chickens were vaccinated with PBS, L. lactis, L. lactis HA1, L. lactis HA1-Fc and inactivated H9N2 vaccine, respectively, then intestinal tissues were collected. IL-2 (A), IFN-γ (B), and IL-4 (C) were detected via real-time PCR. The data were showed as means ± SD (n = 5). ΔΔP < 0.01 relative to PBS. **P < 0.01 relative to PBS and L. lactis. ##P < 0.01 relative to L. lactis HA1. $$P < 0.01 relative to L. lactis HA1-Fc

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Real-time Polymerase Chain Reaction

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 6 Weight change of the vaccinated chickens after challenge with H9N2 avian influenza virus. Chickens were immunized with PBS, L. lactis, L. lactis HA1, L. lactis HA1-Fc and inactivated H9N2 vaccine. 10 days after the boost immunization, the immunized chickens were challenged with H9N2 influenza virus. The weight loss was recorded daily for 2 weeks

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Virus

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 7 Viral shedding from cloacal and oropharyngeal swabs. Immunized chickens were infected with H9N2 avian influenza virus, cloacal and oropharyngeal swabs were collected from challenged chickens on days 2, 4, and 7 post-challenges. Viral titers were detected from oropharyngeal swabs collected from challenged chickens on days 2 (A), 4 (B), and 7 (C) post-challenges. Viral titers were detected from cloacal swabs collected from challenged chickens on days 2 (D), 4 (E), and 7 (F) post-challenges

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Infection, Virus

Journal: Virology journal

Article Title: Vaccination with recombinant Lactococcus lactis expressing HA1-IgY Fc fusion protein provides protective mucosal immunity against H9N2 avian influenza virus in chickens.

doi: 10.1186/s12985-023-02044-9

Figure Lengend Snippet: Fig. 8 Protective effect of L. lactis HA1-Fc on lung injury induced by H9N2 influenza virus infection. Seven days after challenge with H9N2 avian influenza virus, lung tissue of PBS group (A), L. lactis group (B), L. lactis HA1 group (C), L. lactis HA1-Fc group (D) and H9N2 inactivated vaccine (E) was evaluated by histopathological analysis. The sections were stained with H&E. Objective magnification, × 200 (A–E). (F) The virus titers of lung samples from vaccinated chickens were obtained at 7 days after challenge and infectivity was measured by EID50. The data were showed as means ± SD (n = 5). **P < 0.01 relative to PBS and L. lactis. ##P < 0.01 relative to L. lactis HA1

Article Snippet: The presence of the fusion protein was detected using rabbit polyclonal to Avian Influenza A H9N2 Hemagglutinin antibody (Novus Biologicals) followed by horseradish peroxidase-labeled goat anti-rabbit IgG (abcam).

Techniques: Virus, Infection, Staining